Total Fat

Background:

This analysis is used as a determination of the amount of total, or crude, fat in a sample, calculated and expressed in %.

Method

AOAC 991.36-Soxtec Solvent Extraction ISO Accredited

Equipment

Soxtec

Turn Around Time

5 Business Days

Sample Required

About 3 grams is analyzed from a homogenized sample

Type of Test

Solvent Extraction

Reportable Units

% Fat

Detection Limit

Lower Detection Limit: 0.1 % Fat

Analysis Description:

Petroleum ether, a solvent, is used to extract soluble matter, crude fat, from samples of various, complex matrices.

Samples to be analyzed are weighted into thimbles and inserted into the Extraction Unit. After solvent addition to the extraction cups, the soluble material is extracted into the solvent in a two stage processed followed by solvent recovery cycle. Finally the extraction cups are dried and weighed, and a percentage of the total fat in the sample is calculated and reported.